Article
Targeted mutation of barley (1,3;1,4)-β-glucan synthases reveals complex relationships between the storage and cell wall polysaccharide content.
The Plant journal : for cell and molecular biology - 1 Nov 2020
Garcia-Gimenez Guillermo, Barakate Abdellah, Smith Pauline, Stephens Jennifer, Khor Shi F, Doblin Monika S, Hao Pengfei, Bacic Antony, Fincher Geoffrey B, Burton Rachel A, Waugh Robbie, Tucker Matthew R, Houston Kelly
Abstract excerpt
Barley (Hordeum vulgare L) grain is comparatively rich in (1,3;1,4)-β-glucan, a source of fermentable dietary fibre that protects against various human health conditions. However, low grain (1,3;1,4)-β-glucan content is preferred for brewing and distilling. We took a reverse genetics approach, using CRISPR/Cas9 to generate mutations in members of the Cellulose synthase-like (Csl) gene superfamily that encode...
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