Article
Enhancing Catalytic Efficiency of an Actinoplanes utahensis Echinocandin B Deacylase through Random Mutagenesis and Site-Directed Mutagenesis.
Applied biochemistry and biotechnology - 1 Apr 2020
Cheng Ying-Nan, Qiu Shuai, Cheng Feng, Weng Chun-Yue, Wang Ya-Jun, Zheng Yu-Guo
Abstract excerpt
Echinocandin B deacylase (EBDA), from Actinoplanes utahensis ZJB-08196, is capable of cleaving the linoleoyl group from echinocandin B (ECB), forming the echinocandin B nucleus (ECBN), which is a key precursor of semisynthetic antifungal antibiotics. In the present study, molecular evolution of AuEBDA by random mutagenesis combined with site-directed mutagenesis (SDM) and screening was performed. Random...
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