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Article

An engineered old yellow enzyme that enables efficient synthesis of (4R,6R)-Actinol in a one-pot reduction system.

Chembiochem : a European journal of chemical biology - 9 Feb 2015

Horita Shoichiro, Kataoka Michihiko, Kitamura Nahoko, Nakagawa Takuya, Miyakawa Takuya, Ohtsuka Jun, Nagata Koji, Shimizu Sakayu, Tanokura Masaru

Abstract excerpt

(4R,6R)-Actinol can be stereo-selectively synthesized from ketoisophorone by a two-step conversion using a mixture of two enzymes: Candida macedoniensis old yellow enzyme (CmOYE) and Corynebacterium aquaticum (6R)-levodione reductase. However, (4S)-phorenol, an intermediate, accumulates because of the limited substrate range of CmOYE. To address this issue, we solved crystal structures of CmOYE in the presence...

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PMID: 25639703
DOI: 10.1002/cbic.201402555

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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