Understanding the glucose tolerance of an archaeon β-glucosidase from Thermococcus sp.

Abstract excerpt

β-glucosidase hydrolyzes the β-1,4 linkage of cellobiose, a product generated from the action of endoglucanase and cellobiohydrolase on cellulose, and generates glucose. Accumulated glucose during saccharification leads to product inhibition of β-glucosidase, which in turn cause an accumulation of cellobiose and inhibition of other cellulolytic enzymes. Thus, glucose tolerant and active β-glucosidase is required...

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