Article
Mutations at the cysteine codons of the recA gene of Escherichia coli.
DNA (Mary Ann Liebert, Inc.) - 1 Jan 2000
Weisemann J M, Weinstock G M
Abstract excerpt
Each of the three cysteine residues in the Escherichia coli RecA protein was replaced with a number of other amino acids. To do this, each cysteine codon was first converted to a chain-terminating amber codon by oligonucleotide-directed mutagenesis. These amber mutants were then either assayed for function in different suppressor strains or reverted by a second round of mutagenesis with oligonucleotides that had...
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