Article
Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9-1-1 complex in the maintenance of the plastic genome of Leishmania.
Nucleic acids research - 14 Dec 2018
Damasceno Jeziel D, Obonaga Ricardo, Silva Gabriel L A, Reis-Cunha João L, Duncan Samuel M, Bartholomeu Daniella C, Mottram Jeremy C, McCulloch Richard, Tosi Luiz R O
Abstract excerpt
Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene deletion to show that HUS1, a component of the 9-1-1 (RAD9-RAD1-HUS1) complex, is essential and...
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