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Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture

19 Oct 2018

Abstract excerpt

The current expansion of autologous human keratinocytes to resurface severe wound defects still relies on murine feeder layer and calf serum in the cell culture system. Through our characterization efforts of the human skin basement membrane and murine feeder layer 3T3-J2, we identified two biologically relevant recombinant laminins-LN-511 and LN-421- as potential candidates to replace the murine feeder. Herein,...

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PMID: 30377295
DOI: 10.1038/s41467-018-06934-3

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