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Article

Requisite instruments for the establishment of three‐dimensional epidermal human skin equivalents—A methods review

22 Aug 2023

Abstract excerpt

Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review gives an overview of common techniques for setting up HSEs. The HSE consists of an artificial dermis and epidermis. 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts, together with other components, for example, collagen type I, are used to build the...

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PMID: 37605856
DOI: 10.1111/exd.14911

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