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Human epidermis organotypic cultures, a reproducible system recapitulating the epidermis in vitro

28 Apr 2023

Abstract excerpt

The translatability of research is highly dependent on models that recapitulate human tissues and organs. Here, we describe a procedure for the generation of human epidermis organotypic cultures (HEOCs) from primary keratinocytes isolated from foreskin and adult skin as well as from an immortalized keratinocyte cell line (KerTr). We tested several media conditions to develop a defined HEOC growing and expansion...

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PMID: 37114406
DOI: 10.1111/exd.14823

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