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Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

25 Sept 2018

Abstract excerpt

Background: Nucleic acid tests offer powerful and promising tools to strengthen the potential of tuberculosis diagnosis assays. The sensitivity of molecular assays is critical for detection of Mycobacterium tuberculosis (MTB) in paucibacillary sputum. Material and Methods: The impact of targeting repetitive IS6110 sequences on the PCR sensitivity was evaluated across mycobacterium strains and reference material....

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PMID: 30319564
DOI: 10.3389/fmicb.2018.02224

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