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Highly sensitive sequence specific qPCR detection of Mycobacterium tuberculosis complex in respiratory specimens

12 Sept 2016

Abstract excerpt

Nucleic acid amplification tests for Mycobacterium tuberculosis (MTB) detection from sputum are highly sensitive and specific with smear microscopy positive specimens, but their sensitivity with smear-negative/culture-positive specimens is much lower; therefore, these tests cannot rule out a tuberculosis diagnosis. Co-extraction of PCR inhibitors may be a cause of decreased test sensitivity. Here the design and...

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PMID: 27865380
DOI: 10.1016/j.tube.2016.09.002

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