Article
Separation of oligonucleotides with single-base mutation by capillary electrophoresis using specific interaction of metal ion with nucleotide.
The Analyst - 1 Jul 2009
Takahashi Toru, Sakurai Takao, Hirata Koichiro, Hoshino Hitoshi
Abstract excerpt
A unique tactic for the separation of single-base sequential isomers of oligomeric single-stranded DNA by a CE separation system employing the specific interaction of metal ion with nucleotide was demonstrated, enabling the separation of the mixture of a 12-mer oligonucleotide and its single-base mutants, as well as their positional isomers.
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