Article
A transient post-translational modification of active site cysteine alters binding properties of the parkinsonism protein DJ-1.
Biochemical and biophysical research communications - 26 Sept 2018
Mussakhmetov Arman, Shumilin Igor A, Nugmanova Raushan, Shabalin Ivan G, Baizhumanov Timur, Toibazar Daulet, Khassenov Bekbolat, Minor Wladek, Utepbergenov Darkhan
Abstract excerpt
Mutations in the human protein DJ-1 cause early onset of Parkinson's disease. A reactive cysteine residue (Cys106) of DJ-1 is crucial for its protective function, although the underlying mechanisms are unclear. Here we show that a fraction of bacterially expressed polyhistidine-tagged human DJ-1 could not be eluted from a Ni-nitrilotriacetate (Ni-NTA) column with 150 mM imidazole. This unusually tight binding was...
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