Article
Assaying Mutants of Clathrin-Mediated Endocytosis in the Fly Eye.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Lauwers Elsa, Verstreken Patrik
Abstract excerpt
Clathrin-mediated endocytosis plays essential roles both during and after development, and loss-of-function mutants affected in this process are mostly not viable. Different approaches have been developed to circumvent this limitation, including resorting to mosaic model organisms. We here describe the use of FLP/FRT-mediated mitotic recombination to generate Drosophila melanogaster having homozygous mutant eyes...
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