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Article

Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses.

BMC cell biology - 15 Aug 2018

Moorefield Emily C, Blue R Eric, Quinney Nancy L, Gentzsch Martina, Ding Shengli

Abstract excerpt

BACKGROUND: Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to mouse small intestine to create 2D mouse intestinal epithelial monolayers (IEC monolayers) from genetic mouse models for functional analysis. RESULTS: IEC monolayers form epithelial colonies that...

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Identifiers

PMID: 30111276
DOI: 10.1186/s12860-018-0165-0
PMCID: PMC6094565

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CFTR

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