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Article

Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice.

Experimental animals - 26 Feb 2019

Oura Seiya, Miyata Haruhiko, Noda Taichi, Shimada Keisuke, Matsumura Takafumi, Morohoshi Akane, Isotani Ayako, Ikawa Masahito

Abstract excerpt

The CRISPR/Cas9 system can efficiently introduce biallelic mutations in ES cells (ESCs), and its application with fluorescently-tagged ESCs enables phenotype analysis in chimeric mice. We have utilized ESCs that express EGFP in the cytosol and acrosome [EGR-G101 129S2 × (CAG/Acr-EGFP) B6] in previous studies; however, the EGFP signal in the sperm cytosol is weak and the signal in the acrosome is lost after the...

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Identifiers

PMID: 30089752
DOI: 10.1538/expanim.18-0071
PMCID: PMC6389518

Genes

HYDIN

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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