Article
A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
Genesis (New York, N.Y. : 2000) - 1 Jul 2011
Shioi Go, Kiyonari Hiroshi, Abe Takaya, Nakao Kazuki, Fujimori Toshihiko, Jang Chuan-Wei, Huang Cheng-Chiu, Akiyama Haruhiko, Behringer Richard R, Aizawa Shinichi
Abstract excerpt
Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and...
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