Article
Evaluating the accuracy and sensitivity of detecting minority HIV-1 populations by Illumina next-generation sequencing.
Journal of virological methods - 1 Nov 2018
Kireev D E, Lopatukhin A E, Murzakova A V, Pimkina E V, Speranskaya A S, Neverov A D, Fedonin G G, Fantin Yu S, Shipulin G A
Abstract excerpt
The accuracy and sensitivity of deep sequencing were assessed using viral standards (pNL4-3 and pLAI.2) of both DNA and RNA. The sequencing accuracy did not depend on the type of nucleic acid, but critically depended on the number of reads and threshold of sensitivity to minor viral populations. With coverage of more than 236 reads, the accuracy of viral RNA sequencing was equal to or exceeded 99.9%, with a...
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