Article

Accurate bulk quantitation of droplet digital PCR

2021-01-15

Abstract excerpt

Droplet digital PCR provides superior accuracy in nucleic acid quantitation. The requirement of microfluidics to generate and analyze the emulsions, however, is a barrier to its adoption, particularly in low resource or clinical settings. Here, we report a novel method to prepare ddPCR droplets by vortexing and readout the results by bulk analysis of recovered amplicons. We demonstrate the approach by accurately q...

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Literature Corpus work: b90132a8-38cd-5ceb-9040-cdc52731a19d
DOI: 10.1101/2021.01.13.424628

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