Article
Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae.
Journal of microbiological methods - 1 Sept 2013
Nakano Ryuichi, Okamoto Ryoichi, Nakano Akiyo, Nagano Noriyuki, Abe Michiko, Tansho-Nagakawa Shigeru, Ubagai Tsuneyuki, Kikuchi-Ueda Takane, Koshio Osamu, Kikuchi Hirotoshi, Ono Yasuo
Abstract excerpt
We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
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