Article
The comparison of MAMA PCR and SSCP PCR to study chromosomal resistance against Ciprofloxacin and Nalidixic acid in Escherichia coli and Klebsiella pneumoniae.
Microbial pathogenesis - 1 Jul 2018
Hashemi Behnam, Abdollahi Maryam, Rafiei Alireza, Pormohammad Ali, Ahanjan Mohammad, Moghadaszadeh Masoud, Rashidian Shima
Abstract excerpt
The mutation in gyrA and parC genes alters amino acids. Also, it causes resistance against Fluoroquinolones in E. coli and K. pneumoniae. The purpose of this study was to diagnose the significant mutation of gyrA (ser83-asp87) and parC (ser80-glu84) genes through using MAMA PCR and SSCP PCR methods. In so doing, the isolated samples were collected. Then, utilizing agar disc diffusion method, the researchers...
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