Article
A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes.
PloS one - 1 Jan 2018
Koganebuchi Kae, Gakuhari Takashi, Takeshima Hirohiko, Sato Kimitoshi, Fujii Kiyotaka, Kumabe Toshihiro, Kasagi Satoshi, Sato Takehiro, Tajima Atsushi, Shibata Hiroki, Ogawa Motoyuki, Oota Hiroki
Abstract excerpt
To analyze a specific genome region using next-generation sequencing technologies, the enrichment of DNA libraries with targeted capture methods has been standardized. For enrichment of mitochondrial genome, a previous study developed an original targeted capture method that use baits constructed from long-range polymerase chain reaction (PCR) amplicons, common laboratory reagents, and equipment. In this study, a...
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