Article
Low cycle number multiplex PCR: A novel strategy for the construction of amplicon libraries for next-generation sequencing.
Electrophoresis - 1 Aug 2024
Lu Meng, Sun Xiuxiu, Zhao Yuxin, Zheng Linlin, Lin Junjie, Tang Chen, Chao Kaiyue, Chen Ye, Li Kai, Zhou Yuxun, Xiao Junhua
Abstract excerpt
Multiplex PCR is a critical step when preparing amplicon library for next-generation sequencing. However, there are several challenges related to multiplex PCR including poor uniformity, nonspecific amplification, and primer-dimers. To address these issues, we propose a novel solution strategy that involves using a low cycle number (<10 cycles) in multiplex PCR and then employing carrier DNAs and magnetic beads...
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