Article
A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening.
BMC genomics - 6 Dec 2010
You Frank M, Luo Ming-Cheng, Xu Kenong, Deal Karin R, Anderson Olin D, Dvorak Jan
Abstract excerpt
BACKGROUND: A five-dimensional (5-D) clone pooling strategy for screening of bacterial artificial chromosome (BAC) clones with molecular markers utilizing highly-parallel Illumina GoldenGate assays and PCR facilitates high-throughput BAC clone and BAC contig anchoring on a genetic map. However, t...
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