Article
Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case.
Plant & cell physiology - 1 Dec 2003
Lee Shinyoung, Kim Joonyul, Son Jun-Seock, Nam Jongmin, Jeong Dong-Hoon, Lee Keunsub, Jang Seonghoe, Yoo Jihye, Lee Jinwon, Lee Dong-Yeon, Kang Hong-Gyu, An Gynheung
Abstract excerpt
We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in control...
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