Article
Repeat subtraction-mediated sequence capture from a complex genome.
The Plant journal : for cell and molecular biology - 1 Jun 2010
Fu Yan, Springer Nathan M, Gerhardt Daniel J, Ying Kai, Yeh Cheng-Ting, Wu Wei, Swanson-Wagner Ruth, D'Ascenzo Mark, Millard Tracy, Freeberg Lindsay, Aoyama Natsuyo, Kitzman Jacob, Burgess Daniel, Richmond Todd, Albert Thomas J, Barbazuk W Brad, Jeddeloh Jeffrey A, Schnable Patrick S
Abstract excerpt
Sequence capture technologies, pioneered in mammalian genomes, enable the resequencing of targeted genomic regions. Most capture protocols require blocking DNA, the production of which in large quantities can prove challenging. A blocker-free, two-stage capture protocol was developed using Nimble...
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