Article
T315I mutation of BCR-ABL1 into human Philadelphia chromosome-positive leukemia cell lines by homologous recombination using the CRISPR/Cas9 system.
Scientific reports - 2 Jul 2018
Tamai Minori, Inukai Takeshi, Kojika Satoru, Abe Masako, Kagami Keiko, Harama Daisuke, Shinohara Tamao, Watanabe Atsushi, Oshiro Hiroko, Akahane Koshi, Goi Kumiko, Sugihara Eiji, Nakada Shinichiro, Sugita Kanji
Abstract excerpt
In many cancers, somatic mutations confer tumorigenesis and drug-resistance. The recently established clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a potentially elegant approach to functionally evaluate mutations in cancers. To reproduce mutations by homologous recombination (HR), the HR pathway must be functional, but DNA damage repair is frequently impaired in cancers....
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