Article
A new dual-targeting real-time RT-PCR assay for hepatitis D virus RNA detection.
Diagnostic microbiology and infectious disease - 1 Oct 2018
Wang Yan, Glenn Jeffrey S, Winters Mark A, Shen Li-Ping, Choong Ingrid, Shi Ya-Lun, Bi Sheng-Li, Ma Li-Ying, Zeng Hui, Zhang Fu-Jie
Abstract excerpt
In this study, a real-time reverse transcription-polymerase chain reaction (real time RT-PCR) assay targeting 2 genetic segments was established to detect HDV RNA. Utilizing the World Health Organization International Standard for Hepatitis D Virus RNA, the lower limit of detection was 575 IU/mL, and the linearity of quantification ranged from 575,000 IU/mL to 575 IU/mL. 384 HBsAg-positive samples collected from...
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