Article
Rapid detection of duck hepatitis A virus genotype C using reverse transcription loop-mediated isothermal amplification.
Journal of virological methods - 1 Feb 2014
Li Chuanfeng, Chen Zongyan, Meng Chunchun, Liu Guangqing
Abstract excerpt
A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was used and optimized to develop a rapid and sensitive detection system for duck hepatitis A virus genotype C (DHAV-C) RNA. A set of four specific primers was designed against highly conserved sequences located within the 3D gene from DHAV (strain GX1201). Under optimal reaction conditions, the sensitivity of DHAV-C-specific...
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