Article
Solid-state NMR analysis of the sodium pump Krokinobacter rhodopsin 2 and its H30A mutant.
Journal of structural biology - 1 Apr 2019
Kaur Jagdeep, Kriebel Clara Nassrin, Eberhardt Peter, Jakdetchai Orawan, Leeder Alexander J, Weber Ingrid, Brown Lynda J, Brown Richard C D, Becker-Baldus Johanna, Bamann Christian, Wachtveitl Josef, Glaubitz Clemens
Abstract excerpt
Krokinobacter eikastus rhodopsin 2 (KR2) is a pentameric, light-driven ion pump, which selectively transports sodium or protons. The mechanism of ion selectivity and transfer is unknown. By using conventional as well as dynamic nuclear polarization (DNP)-enhanced solid-state NMR, we were able to analyse the retinal polyene chain between positions C10 and C15 as well as the Schiff base nitrogen in the KR2 resting...
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