McSpoligotyping, a One-Step Melting Curve Analysis-Based Protocol for Spoligotyping of Mycobacterium tuberculosis.

Abstract excerpt

The direct repeat (DR) region in the Mycobacterium tuberculosis (MTB) genome is composed of highly polymorphic direct variant repeats, which are the basis of spacer oligonucleotide typing (spoligotyping) to study the population structure and epidemiology of M. tuberculosis However, the membrane hybridization-based detection format requires various post-PCR manipulations and is prone to carryover contamination,...

Topics

• DNA, Bacterial
• Genotype
• Humans
• Molecular Epidemiology
• Molecular Typing
• Mycobacterium tuberculosis
• Polymorphism, Single Nucleotide
• Real-Time Polymerase Chain Reaction
• Repetitive Sequences, Nucleic Acid
• Sequence Analysis, DNA
• Time Factors
• Tuberculosis