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Article

Single-tube protocol for culture-independent spoligotyping of Mycobacterium tuberculosis based on MeltArray.

Journal of clinical microbiology - 17 Jan 2024

Xia Zihan, Su Biyi, Tu Chunxia, Sun Siyang, Tan Yaoju, Xu Ye, Li Qingge

Abstract excerpt

IMPORTANCE: Spacer oligonucleotide typing (spoligotyping), the first-line genotyping assay for Mycobacterium tuberculosis (MTB), plays a fundamental role in the investigation of its epidemiology and evolution. In this study, we established a single-tube spoligotyping assay using MeltArray, a highly multiplex polymerase chain reaction (PCR) approach that runs on a real-time PCR thermocycler. The MeltArray protocol...

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Source

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Identifiers

PMID: 38112521
DOI: 10.1128/jcm.01183-23
PMCID: PMC10793361

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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