Article
Variation in human chromosome 21 ribosomal RNA genes characterized by TAR cloning and long-read sequencing.
Nucleic acids research - 27 Jul 2018
Kim Jung-Hyun, Dilthey Alexander T, Nagaraja Ramaiah, Lee Hee-Sheung, Koren Sergey, Dudekula Dawood, Wood Iii William H, Piao Yulan, Ogurtsov Aleksey Y, Utani Koichi, Noskov Vladimir N, Shabalina Svetlana A, Schlessinger David, Phillippy Adam M, Larionov Vladimir
Abstract excerpt
Despite the key role of the human ribosome in protein biosynthesis, little is known about the extent of sequence variation in ribosomal DNA (rDNA) or its pre-rRNA and rRNA products. We recovered ribosomal DNA segments from a single human chromosome 21 using transformation-associated recombination (TAR) cloning in yeast. Accurate long-read sequencing of 13 isolates covering ∼0.82 Mb of the chromosome 21 rDNA...
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