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Article

Local pH elevation mediated by the intrabacterial urease of Helicobacter pylori cocultured with gastric cells.

The Journal of clinical investigation - 1 Aug 2000

Athmann C, Zeng N, Kang T, Marcus E A, Scott D R, Rektorschek M, Buhmann A, Melchers K, Sachs G

Abstract excerpt

Helicobacter pylori resists gastric acidity by modulating the proton-gated urea channel UreI, allowing for pH(out)-dependent regulation of urea access to intrabacterial urease. We employed pH- and Ca(2+)-sensitive fluorescent dyes and confocal microscopy to determine the location, rate, and magnitude of pH changes in an H. pylori-AGS cell coculture model, comparing wild-type bacteria with nonpolar ureI-deletion...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 10930437
DOI: 10.1172/JCI9351
PMCID: PMC314326

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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