Article
[Effect of Arg188Gln (G/A) mutation on enzymatic activity of kynureninase].
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 25 May 2017
Shen Jie, Chen Wendong, Ji Kaida, Gao Pingjin, Zhu Dingliang
Abstract excerpt
Objective: To verify whether the enzymatic activity of kynureninase (KYNU) could be changed by the Arg188Gln (G/A) mutation. Methods: The total RNA of human hepatic tissue was extracted and the KYNU gene cDNA was amplified by RT-PCR. Primers were designed according to the sequences around the site Arg188Gln of KYNU gene and the Arg188Gln (G/A) mutant KYNU cDNA was generated by site-directed mutagenesis. Both the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.