Article
Site-directed mutagenesis of recombinant rat DNA polymerase beta: involvement of arginine-183 in primer recognition.
Biochemistry - 29 May 1990
Date T, Yamamoto S, Tanihara K, Nishimoto Y, Liu N, Matsukage A
Abstract excerpt
By site-directed mutagenesis using synthetic oligonucleotides, amino acid residues 181Phe-Arg-Arg183 of recombinant rat DNA polymerase beta were replaced by other amino acids to clarify the roles of these residues in the DNA synthesizing reaction. Replacement of Phe-181 by alanine reduced the enz...
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Base Sequence
- DNA Polymerase I
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleic Acid Precursors
- Oligonucleotides
- Rats
- Recombinant Proteins
- Substrate Specificity