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Article

Site-directed mutagenesis of recombinant rat DNA polymerase beta: involvement of arginine-183 in primer recognition.

Biochemistry - 29 May 1990

Date T, Yamamoto S, Tanihara K, Nishimoto Y, Liu N, Matsukage A

Abstract excerpt

By site-directed mutagenesis using synthetic oligonucleotides, amino acid residues 181Phe-Arg-Arg183 of recombinant rat DNA polymerase beta were replaced by other amino acids to clarify the roles of these residues in the DNA synthesizing reaction. Replacement of Phe-181 by alanine reduced the enzyme activity only 30%. Replacement of Arg-182 by alanine and glutamine resulted in reduction of the activity by about...

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PMID: 2198936
DOI: 10.1021/bi00473a005

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