Article
Comparison of ex vivo and in vitro intestinal cystic fibrosis models to measure CFTR-dependent ion channel activity.
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society - 1 May 2018
Zomer-van Ommen Domenique D, de Poel Eyleen, Kruisselbrink Evelien, Oppelaar Hugo, Vonk Annelotte M, Janssens Hettie M, van der Ent Cornelis K, Hagemeijer Marne C, Beekman Jeffrey M
Abstract excerpt
BACKGROUND: New functional assays using primary human intestinal adult stem cell cultures can be valuable tools to study epithelial defects in human diseases such as cystic fibrosis. METHODS: CFTR-mediated ion transport was measured in rectal organoid-derived monolayers grown from subjects with various CFTR mutations and compared to donor-matched intestinal current measurements (ICM) in rectal biopsies and...
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