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Article

Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9.

PloS one - 1 Jan 2018

Honma Kazuhisa, Abe Satoshi, Endo Takeshi, Uno Narumi, Oshimura Mitsuo, Ohbayashi Tetsuya, Kazuki Yasuhiro

Abstract excerpt

Mouse artificial chromosome (MAC) vectors have several advantages as gene delivery vectors, such as stable and independent maintenance in host cells without integration, transferability from donor cells to recipient cells via microcell-mediated chromosome transfer (MMCT), and the potential for loading a megabase-sized DNA fragment. Previously, a MAC containing a multi-integrase platform (MI-MAC) was developed to...

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Identifiers

PMID: 29505588
DOI: 10.1371/journal.pone.0193642
PMCID: PMC5837097

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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