Article
A comparison of CRISPR/Cas9 and siRNA-mediated ALDH2 gene silencing in human cell lines.
Molecular genetics and genomics : MGG - 1 Jun 2018
Wang Fei, Guo Tao, Jiang Hongmei, Li Ruobi, Wang Ting, Zeng Ni, Dong Guanghui, Zeng Xiaowen, Li Daochuan, Xiao Yongmei, Hu Qiansheng, Chen Wen, Xing Xiumei, Wang Qing
Abstract excerpt
Gene knockdown and knockout using RNAi and CRISPR/Cas9 allow for efficient evaluation of gene function, but it is unclear how the choice of technology can influence the results. To compare the phenotypes obtained using siRNA and CRISPR/Cas9 technologies, aldehyde dehydrogenase 2 (ALDH2) was selected as an example. In this study, we constructed one HepG2 cell line with a homozygous mutation in the fifth exon of...
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