Article
Role of the AP-5 adaptor protein complex in late endosome-to-Golgi retrieval.
PLoS biology - 1 Jan 2018
Hirst Jennifer, Itzhak Daniel N, Antrobus Robin, Borner Georg H H, Robinson Margaret S
Abstract excerpt
The AP-5 adaptor protein complex is presumed to function in membrane traffic, but so far nothing is known about its pathway or its cargo. We have used CRISPR-Cas9 to knock out the AP-5 ζ subunit gene, AP5Z1, in HeLa cells, and then analysed the phenotype by subcellular fractionation profiling and quantitative mass spectrometry. The retromer complex had an altered steady-state distribution in the knockout cells,...
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