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Article

Simultaneous amplification of exons 18 to 21 of the EGFR gene using 5' tailed primers and a two-stage protocol.

Nucleosides, nucleotides & nucleic acids - 2 Jan 2018

Luo Kai, Wang Qian, Qiu Qinwei, Li Xiemengdan, Jia Xiaoting, Chen Xiaoliang, Zhang Zhijie, Zheng Guopei, He Zhiming

Abstract excerpt

OBJECTIVE: Reduction of non-specific amplification and achievement of efficient amplification of multiple gene fragments under the same reaction condition is the basic goal of PCR diagnosis; however, this is often difficult. This study was conducted to establish a highly specific and effective amplification of the epidermal growth factor receptor (EGFR) gene's exons, 18-21, simultaneously. METHODS: The 5'-tailed...

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Identifiers

PMID: 29337648
DOI: 10.1080/15257770.2017.1375517

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EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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