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Article

A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli.

Nature chemical biology - 1 Mar 2018

Donnelly Ann E, Murphy Grant S, Digianantonio Katherine M, Hecht Michael H

Abstract excerpt

Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that...

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PMID: 29334382
DOI: 10.1038/nchembio.2550

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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