Article
Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer.
Science advances - 1 Jan 2018
Bhuripanyo Karan, Wang Yiyang, Liu Xianpeng, Zhou Li, Liu Ruochuan, Duong Duc, Zhao Bo, Bi Yingtao, Zhou Han, Chen Geng, Seyfried Nicholas T, Chazin Walter J, Kiyokawa Hiroaki, Yin Jun
Abstract excerpt
E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins and regulate diverse cellular processes. To profile their ubiquitination targets in the cell, we used phage display to engineer E2-E4B and E2-CHIP pairs that were free of cross-reactivity with the native UB transfer cascades. We then...
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