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Article

Redesigning the NEDD8 pathway with a bacterial genetic screen for ubiquitin-like molecule transfer.

Journal of molecular biology - 4 May 2012

Guntas Gurkan, Kuhlman Brian

Abstract excerpt

Pathways of ubiquitin-like (UBL) molecule transfer regulate a myriad of cellular cascades. Here, we report a high-throughput assay that correlates catalytic human NEDD8 transfer to bacterial survival. The assay was utilized to screen mutant NEDD8 and NAE (NEDD8-activating enzyme) libraries to engineer a more stable NEDD8 and redesign the NEDD8-NAE interface. This approach will be useful in understanding the...

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Source

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Identifiers

PMID: 22391419
DOI: 10.1016/j.jmb.2012.02.036
PMCID: PMC3329750

Genes

NEDD8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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