Article
Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types.
Journal of medical virology - 1 May 2018
Okodo Mitsuaki, Okayama Kaori, Teruya Koji, Sasagawa Toshiyuki
Abstract excerpt
We have developed a new human papillomavirus (HPV) assay using the uniplex E6/E7 polymerase chain reaction (PCR) method, which is able to detect E6 and E7 genes in 39 HPV types. We validated the assay for sensitivity and specificity using cloned HPV DNA and clinical samples. A comparative study using Genosearch-31 (GS-31) to determine HPV genotypes in clinical samples was also performed. E6 or E7 genes, measured...
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