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Article

Detection and typing of human papillomavirus by e6 nested multiplex PCR.

Journal of clinical microbiology - 1 Jul 2004

Sotlar K, Diemer D, Dethleffs A, Hack Y, Stubner A, Vollmer N, Menton S, Menton M, Dietz K, Wallwiener D, Kandolf R, Bültmann B

Abstract excerpt

A nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and type-specific primers was evaluated for the detection and typing of human papillomavirus (HPV) genotypes 6/11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 56, 58, 59, 66, and 68 using HPV DNA-containing plasmids and cervical scrapes (n = 1,525). The performance of the NMPCR assay relative to that of conventional PCR with...

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Identifiers

PMID: 15243079
DOI: 10.1128/JCM.42.7.3176-3184.2004
PMCID: PMC446280

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Source receipt: pubmed, retrieved 7 Sept 2026.

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