Article
Detection and typing of human papillomavirus by e6 nested multiplex PCR.
Journal of clinical microbiology - 1 Jul 2004
Sotlar K, Diemer D, Dethleffs A, Hack Y, Stubner A, Vollmer N, Menton S, Menton M, Dietz K, Wallwiener D, Kandolf R, Bültmann B
Abstract excerpt
A nested multiplex PCR (NMPCR) assay that combines degenerate E6/E7 consensus primers and type-specific primers was evaluated for the detection and typing of human papillomavirus (HPV) genotypes 6/11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 51, 52, 56, 58, 59, 66, and 68 using HPV DNA-containing plasmids and cervical scrapes (n = 1,525). The performance of the NMPCR assay relative to that of conventional PCR with...
Topics
- DNA Primers
- DNA, Viral
- Female
- Genotype
- Humans
- Oncogene Proteins, Viral
- Papillomaviridae
- Polymerase Chain Reaction
- Sensitivity and Specificity