Article
Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells.
Methods (San Diego, Calif.) - 1 May 2018
Davey Rhonda J, Digman Michelle A, Gratton Enrico, Moens Pierre D J
Abstract excerpt
Image mean square displacement analysis (iMSD) is a method allowing the mapping of diffusion dynamics of molecules in living cells. However, it can also be used to obtain quantitative information on the diffusion processes of fluorescently labelled molecules and how their diffusion dynamics change when the cell environment is modified. In this paper, we describe the use of iMSD to obtain quantitative data of the...
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