Article
2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Wessels Deborah, Kuhl Spencer, Soll David R
Abstract excerpt
2D- and 3D-Dynamic Image Analysis Systems (2D- and 3D-DIAS) for quantitative analysis of cell motility and chemotaxis are described. Particular attention is given to protocols that have proven useful in the quantitation of cell shape changes and pseudopod dynamics during basic cell motility (i.e. crawling in the absence of a chemotactic or other type of extracellular signal) and directed motion. In addition,...
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