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Article

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration.

Plant biotechnology journal - 1 Jul 2018

Lin Choun-Sea, Hsu Chen-Tran, Yang Ling-Hung, Lee Lan-Ying, Fu Jin-Yuan, Cheng Qiao-Wei, Wu Fu-Hui, Hsiao Han C-W, Zhang Yesheng, Zhang Ru, Chang Wan-Jung, Yu Chen-Ting, Wang Wen, Liao Li-Jen, Gelvin Stanton B, Shih Ming-Che

Abstract excerpt

Plant protoplasts are useful for assessing the efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mutagenesis. We improved the process of protoplast isolation and transfection of several plant species. We also developed a method to isolate and regenerate single mutagenized Nicotianna tabacum protoplasts into mature plants. Following transfection of...

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Identifiers

PMID: 29230929
DOI: 10.1111/pbi.12870
PMCID: PMC5999315

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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