Efficient and Heritable Targeted Mutagenesis in Mosses Using the CRISPR/Cas9 System.

Abstract excerpt

Targeted genome modification by RNA-guided nucleases derived from the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system has seen rapid development in many organisms, including several plant species. In the present study, we succeeded in introducing the CRISPR/Cas9 system into the non-model organism Scopelophila cataractae, a moss that exhibits heavy metal...

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• Endonucleases
• Gene Editing
• Mutagenesis, Site-Directed